Part:BBa_K1422506

Plasmid producing corn NAAT1, DMAS, and TOM1

This plasmid codes for NAAT1 (BBa_K1422502) and DMAS (BBa_K1422503), two of the four enzymes used to produce corn phytosiderophores; TOM1 (BBa_K1422507), which transports phytosiderophores out of cells; and sfGFP, as a reporter. BBa_K1422505 goes along with this plasmid, producing the other two enzymes necessary for the production of corn phytosiderophores, and includes a killswitch.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 2786
  Illegal EcoRI site found at 2800
  Illegal XbaI site found at 1434
  Illegal XbaI site found at 3442
  Illegal SpeI site found at 939
  Illegal PstI site found at 1019
  Illegal PstI site found at 1893
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 2786
  Illegal EcoRI site found at 2800
  Illegal NheI site found at 201
  Illegal NheI site found at 1249
  Illegal SpeI site found at 939
  Illegal PstI site found at 1019
  Illegal PstI site found at 1893
  Illegal NotI site found at 349
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 2786
  Illegal EcoRI site found at 2800
  Illegal BglII site found at 1463
  Illegal BglII site found at 3158
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 2786
  Illegal EcoRI site found at 2800
  Illegal XbaI site found at 1434
  Illegal XbaI site found at 3442
  Illegal SpeI site found at 939
  Illegal PstI site found at 1019
  Illegal PstI site found at 1893
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 2786
  Illegal EcoRI site found at 2800
  Illegal XbaI site found at 1434
  Illegal XbaI site found at 3442
  Illegal SpeI site found at 939
  Illegal PstI site found at 1019
  Illegal PstI site found at 1893
  Illegal NgoMIV site found at 1990
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 3649
  Illegal BsaI.rc site found at 4022
  Illegal SapI.rc site found at 2636
  Illegal SapI.rc site found at 4158